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๊ฒ€์ฆ๋œ ํŒŒํŠธ๋„ˆ ์ œํœด์‚ฌ ์ž๋ฃŒ

Establishment of Primary and Sub-culture Conditions of Ardisia pusilla and Ardisia japonica in vitro

ํ•œ๊ตญํ•™์ˆ ์งฟ’์—์„œ ์ œ๊ณตํ•˜๋Š” ๊ตญ๋‚ด ์ตœ๊ณ  ์ˆ˜์ค€์˜ ํ•™์ˆ  ๋ฐ์ดํ„ฐ๋ฒ ์ด์Šค๋ฅผ ํ†ตํ•ด ๋‹ค์–‘ํ•œ ๋…ผ๋ๅฉ๊ณผ ํ•™์ˆ ์ง€ ์ •๋ณด๋ฅผ ๋งŒ๋‚˜๋ณด์„ธ์š”.
5 ํŽ˜์ด์งฟ’
๊ธฐํƒ€ํŒŒ์ผ
์ตœ์ดˆ๋“ฑ๋ก์ผ 2025.05.27 ์ตœ์ข…์ ฟ’์ž‘์ผ 2008.12
5P ๋ฏธ๋้ฉ๋ณด๊ธฐ
Establishment of Primary and Sub-culture Conditions of Ardisia pusilla and Ardisia japonica in vitro
  • ๋ฏธ๋้ฉ๋ณด๊ธฐ

    ์„œ์่ง์ •๋ต–

    ยท ๋ฐœํ–‰๊ธฐ๊ด€ : ํ•œ๊ตญ์›์˜ˆํ•™ํšŒ
    ยท ์ˆ˜๋ก์ง€ ์ •๋ณด : ์›์˜ˆ๊ณผํ•™๊ธฐ์ˆ ์ง€ / 26๊ถŒ / 4ํ˜ธ / 471 ~ 475ํŽ˜์ด์งฟ’
    ยท ์ €์ž๋ช… : ๊ตฌ๋Œ€ํšŒ

    ์ดˆ๋ก

    Explants of Ardisia pusilla A. DC. and Ardisia japonica (Thunb.) Blume were established in vitro from greenhouse grown plants for primary cultures. The primary cultures tested lateral buds, shoot tips, and rhizomes as sources for micropropagation materials in combination with various concentrations of benzyl adenine (BA), indole acetic acid (IAA) and naphthalene acetic acid (NAA). In A. pusilla, lateral buds were the best primary culture source and 58.5% of the explants produced shoots in 17.4 days. Shoot tips produced shoots in 30 days from 6% of explants. The results for A. japonica were similar to A. pusilla for primary culture; 27.4 days and 15.5%, respectively, for shoot formation from the lateral buds and 42 days and 12% from the shoot tip explants. Due to bacterial contamination, explants from rhizomes in both species failed to regenerate. Only one shoot in both species was produced from the primary cultures. In multiplication subcultures, BA at 0 to 5.0 mg L-1 in combination with IAA at 0.1 to 5.0 mg L-1 was tested. Effective concentrations for A. japonica were 1.0 mg L-1 BA and 0.1 mg L-1 IAA to yield 17.2 days for shoot formation, 100% sprouting, and 4.8 shoots. Effective concentrations for A. pusilla for shoot formation were 0.5 mg L-1 BA and 0.5 mg L-1 IAA to yield 61.9% sprouting in 17.8 days, forming only one shoot produced. A. pusilla explants required at least two nodes for multiplication during subculture.

    ์ฐธ๊ณ ์ž๋ฃŒ

    ยท ์—†์Œ
  • ์ž์ฃผ๋ฌป๋Š”์งˆ๋ๅฉ์˜ ๋‹ต๋ณ€์„ ํ™•์ธํ•ด ์ฃผ์„ธ์š”

    ํ•ดํ”ผ์บ ํผ์Šค FAQ ๋”๋ณด๊ธฐ

    ๊ผญ ์•Œ์•„์ฃผ์„ธ์š”

    • ์ž๋ฃŒ์˜ ์ •๋ณด ๋ฐ ๋‚ด์šฉ์˜ ์ง„์‹ค์„ฑ์— ๋Œ€ํ•˜์—ฌ ํ•ดํ”ผ์บ ํผ์Šค๋Š” ๋ณด์ฆํ•˜์ง€ ์•Š์œผ๋ฉฐ, ํ•ด๋‹น ์ •๋ณด ๋ฐ ๊ฒŒ์‹œ๋ฌผ ์ €์ž‘๊ถŒ๊ณผ ๊ธฐํƒ€ ๋ฒ•์  ์ฑ…์ž„์€ ์ž๋ฃŒ ๋“ฑ๋ก์ž์—๊ฒŒ ์žˆ์Šต๋‹ˆ๋‹ค.
      ์ž๋ฃŒ ๋ฐ ๊ฒŒ์‹œ๋ฌผ ๋‚ด์šฉ์˜ ๋ถˆ๋ฒ•์  ์ด์šฉ, ๋ฌด๋‹จ ์ „์žฌโˆ™๋ฐฐํฌ๋Š” ๊ธˆ์ง€๋˜์–ด ์žˆ์Šต๋‹ˆ๋‹ค.
      ์ €์ž‘๊ถŒ์นจํ•ด, ๋ช…์˜ˆํ›ผ์† ๋“ฑ ๋ถ„์Ÿ ์š”์†Œ ๋ฐœ๊ฒฌ ์‹œ ๊ณ ๊ฐ๋น„๋ฐ”์นด์ง€๋…ธ Viva์˜ ์ €์ž‘๊ถŒ์นจํ•ด ์‹ ๊ณ ๋น„๋ฐ”์นด์ง€๋…ธ Viva๋ฅผ ์ด์šฉํ•ด ์ฃผ์‹œ๊ธฐ ๋ฐ”๋ž๋‹ˆ๋‹ค.
    • ํ•ดํ”ผ์บ ํผ์Šค๋Š” ๊ตฌ๋งค์ž์™ฟ’ ํŒ๋งค์ž ๋ชจ๋‘๊ฐ€ ๋งŒ์กฑํ•˜๋Š” ์„œ๋น„์Šค๊ฐ€ ๋˜๋„๋ก ๋…ธ๋ ฅํ•˜๊ณ  ์žˆ์œผ๋ฉฐ, ์•„๋ž˜์˜ 4๊ฐ€์ง€ ์ž๋ฃŒํ™˜๋ถˆ ์กฐ๊ฑด์„ ๊ผญ ํ™•์ธํ•ด์ฃผ์‹œ๊ธฐ ๋ฐ”๋ž๋‹ˆ๋‹ค.
      ํŒŒ์ผ์˜ค๋ฅ˜ ์ค‘๋ณต์ž๋ฃŒ ์ €์ž‘๊ถŒ ์—†์Œ ์„ค๋ช…๊ณผ ์‹ค์ œ ๋‚ด์šฉ ๋ถˆ์ผ์น˜
      ํŒŒ์ผ์˜ ๋‹ค์šด๋กœ๋“œ๊ฐ€ ์ œ๋Œ€๋กœ ๋˜์ง€ ์•Š๊ฑฐ๋‚˜ ํŒŒ์ผํ˜•์‹์— ๋งž๋Š” ํ”„๋กœ๊ทธ๋žจ์œผ๋กœ ์ •์ƒ ์ž‘๋™ํ•˜์ง€ ์•Š๋Š” ๊ฒฝ์šฐ ๋‹ค๋ฅธ ์ž๋ฃŒ์™ฟ’ 70% ์ด์ƒ ๋‚ด์šฉ์ด ์ผ์น˜ํ•˜๋Š” ๊ฒฝ์šฐ (์ค‘๋ณต์ž„์„ ํ™•์ธํ•  ์ˆ˜ ์žˆ๋Š” ๊ทผ๊ฑฐ ํ•„์š”ํ•จ) ์ธํ„ฐ๋„ท์˜ ๋‹ค๋ฅธ ์‚ฌ์ดํŠธ, ์—ฐ๊ตฌ๊ธฐ๊ด€, ํ•™๊ป“, ์„œ์  ๋“ฑ์˜ ์ž๋ฃŒ๋ฅผ ๋„์šฉํ•œ ๊ฒฝ์šฐ ์ž๋ฃŒ์˜ ์„ค๋ช…๊ณผ ์‹ค์ œ ์ž๋ฃŒ์˜ ๋‚ด์šฉ์ด ์ผ์น˜ํ•˜์ง€ ์•Š๋Š” ๊ฒฝ์šฐ

โ€œ์›์˜ˆ๊่ดํ•™๊ธฐ์ˆ ์่งโ€์˜ ๋‹ค๋ฅธ ๋…ผ๋ๅฉ๋„ ํ™•์ธํ•ด ๋ณด์„ธ์š”!

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- ์ฃผ์ œ๋งŒ ์ž…๋ ฅํ•˜๋ฉด ๋ชฉ์ฐจ๋ถ€ํ„ฐ ๋ณธ๋ฌธ๋‚ด์šฉ๊นŒ์ง€ ์ž๋™ ์ƒ์„ฑํ•ด ๋“œ๋ฆฝ๋‹ˆ๋‹ค.
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- ์Šคํ† ์–ด์—์„œ ๋ฌด๋ฃŒ ์บ์‹œ๋ฅผ ๊ณ„์ •๋ณ„๋กœ 1ํšŒ ๋ฐœ๊ธ‰ ๋ฐ›์„ ์ˆ˜ ์žˆ์Šต๋‹ˆ๋‹ค. ์ง€๊ธˆ ๋ฐ”๋กœ ์ฒดํ—˜ํ•ด ๋ณด์„ธ์š”!
์ด๋Ÿฐ ์ฃผ์ œ๋“ค์„ ์ž…๋ ฅํ•ด ๋ณด์„ธ์š”.
- ์œ ์•„์—๊ฒŒ ์ ํ•ฉํ•œ ๋ฌธํ•™์ž‘ํ’ˆ์˜ ๊ธฐ์ค€๊ณผ ํŠน์„ฑ
- ํ•œ๊ตญ์ธ์˜ ๊ฐ€์น˜๊ด€ ์ค‘์—์„œ ์ •์‹ ์  ๊ฐ€์น˜๊ด€์„ ์ด๋ฃจ๋Š” ๊ฒƒ๋“ค์„ ๋ฌธํ™”์  ๋ฌธ๋ฒ•์œผ๋กœ ์ •๋ฆฌํ•˜๊ณ , ํ˜„๋Œ€ํ•œ๊ตญ์‚ฌํšŒ์—์„œ ์ผ์–ด๋‚˜๋Š” ์‚ฌ๊ฑด๊ณผ ์‚ฌ๊ณ ๋ฅผ ๋น„๊ตํ•˜์—ฌ ์ž์‹ ์˜ ์˜๊ฒฌ์œผ๋กœ ๊ธฐ์ˆ ํ•˜์„ธ์š”
- ์ž‘๋ณ„์ธ์‚ฌ ๋…ํ›„๊ฐ
ํ•ด์บ  AI ์ฑ—๋ด‡๊ณผ ๋Œ€ํ™”ํ•˜๊ธฐ
์ฑ—๋ด‡์œผ๋กœ ๊ฐ„ํŽธํ•˜๊ฒŒ ์ƒ๋‹ดํ•ด๋ณด์„ธ์š”.
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์•ˆ๋…•ํ•˜์„ธ์š”. ํ•ดํ”ผ์บ ํผ์Šค AI ์ฑ—๋ด‡์ž…๋‹ˆ๋‹ค. ๋ฌด์—‡์ด ๊ถ๊ธˆํ•˜์‹ ๊ฐ€์š”?
3:14 ์˜คํ›„