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This research examined the effects of perceived parental overprotection on interpersonal relationships of university students, whether the mediating effect of shame. Questionnaire survey was conducted on total 208 1st~4th grade university students, who are enrolled in university located in Seoul and Gyeonggi-do. The main results of this study were as follows: First, perceived parental overprotection, interpersonal relationships and shame showed a significant correlation. Second, perceived parental overprotection had negative effect on interpersonal relationships. Third, The higher the degree of shame, the more negative impact on interpersonal relationships. Forth, Shame was found to have a partial mediating effect between perceived parental overprotection and interpersonal relationship. This implies that perceived parental overprotection indirectly influences university students' interpersonal relationships by using shame as a mediator. Based on these findings, the limitations and implications of this research were discussed.