Agarose-gel ?? ?? ?? ???

3. ????
¢Ù 0.8% agarose gel? ???. agarose? 20ml? TAE buffer? ?? ????? ? 1?? ???. (1%(w/v) agarose gel ???, 1 g% = 1 g/100 ml??? TAE Buffer100 ml? 1 g? agarose? ??? ??.)
¢Ú ???? gel? ???? ?(gel cast)? ???? ? 10? ?? ???.(????? ??? ???? ???)
¢Û Parafilm? loading buffer? ??? ? ???? ??? ?? ???. (Loading buffer : DNA sample ??= 1:5)
¢Ü ???? 2?? DNA Sample? loading buffer? ?? ? ?? ??(? 3?)? ?? ???? ????.
¢Ý ?? gel? ?? ???? ?? ?? ??? DNA Sample? Size marker? ???? gel? Well(?? ?? ?)? ?????.
¢Þ Bromophenol blue dye? ??? ????? ??? ????? ????, ??? gel ?? ? ? ??? ?? ??? ??? ??? gel? ???. (DNA Sample? ??? ?? ????? ???? ?? 10~15min??? ???. )
¢ß ??: ?? Gel? EtBr ??? 10?, DDW ? 5?? ???.(EtBr ? DDW? ???.)
¢à ? ?? UV transilluminator?? ???. ???? ?? DNA? ?? ??? ??? ??? ??.
¢á ? ??? ???? ???? DNA? ??? ??? ? ??.

????
¡ù Etidium Bromide? DNA ????? ??? ????? ??? gel? ???? ?? DNA?? ??? ?? ?? ???? ? ?? ??? ??. Etidium Bromide? Mutation? ???? ??????? ???? ?? ????? ?? ? ????? ????.