![[???????] PCR](https://image4.ppt2video.com/Production/thumb212/2017/11/03/data20021191-0001.jpg)
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1. Summary
2. Keywords
3. Introduction
4. Materials & Methods
5. Reference????
Summary
PCR? Denaturation, Annealing, Elongation? 3??? ???. Denaturation? ?? ??? ???? ???? DNA(dsDNA)? ???? DNA(ssDNA)? ?????. Annealing? 50¡æ~65¡æ?? ????. Primer? ??? ????? ???? ????? ?? ?? DNA? ???? ????. Elongation? Taq polymerase? ???? target DNA template? ???? DNA ??? ???? ????. ??? Taq polymerase? Termusaquaticus?? ???? ?? ??? ????, ?? ?? ???? ?? ????. ??? PCR? ???? cDNA? ?? ???? ? ???? 30cycle? ??? ?? 230?? cDNA? ?? ? ??. ??? ??? ?? ?? ??? electrophoresis? ?? ??? ?? O.D? ?? ??? ????. ???? ???? 4?? ??? ? ??? ? 300bp, ? 600bp? band? ????? DNA template ??? 2??? ?????? ? ? ???, ?? 1?? ??? ? ???? Control? 1563.5, Reaction mixture? 1604.0? ???? ? ? ???. ??? ? ?? ? 1.65? purity? ????? ? ??? RT-PCR? ???? buffer, enzyme, dNTP, Oligo dT primer? ???? ????.
Keywords
PCR / Taq polymerase/ DNA template
Introduction
???? ????(polymerase chain reaction, PCR)? ?? DNA ??? ????? ??, ???? ??? ??? ??? DNA ??? ????? ????. ?? ?? ?? DNA? ???? ?? ?? DNA ??? ????? ?? ?????? ????? ???? ???? ???.????
¡¤ http://ko.wikipedia.org/wiki/%EC%A4%91%ED%95%A9%ED%9A%A8%EC%86%8C_%EC%97%B0%EC%87%84_%EB%B0%98%EC%9D%91
¡¤ http://biochemistry.yonsei.ac.kr/biochem_workshop/PCR.php
¡¤ http://www.genehunters.co.kr/Training/01_2.htm
¡¤ http://biochemistry.yonsei.ac.kr/biochem_molecular/gene_cloning_08.php
¡¤ Robert F. Weaver ????? (2?) / ??? ?? / ??????? / p67??
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?????(OM), ???????(SEM), ???????(TEM)? ??
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1. ?? ??? (optical microscope), ¹âŒWï@΢çR ?? ????? ??? ?? ??? ? ??? ??? ?? ????? ?? ??? ??? ??, ??? ????? ?? ???? ?? ??? ? ??? ??? ????, ????? ????? ??? ?? ???? ????. ????? ????, ??(?)? ????? ????? ??? ?.. -
RNA isolation, RTase ??? ?? ??? (????, ??, discussion)
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[?? ??] - PPAR-gamma? ??? ?? ? ??? H2073??, ??? ?? ?? H1993 cell?? ???? ??? ? ??? ?? ?? [?? ??- RNA isolation] <HER2 (Human Epidermal growth factor Receptor 2)> - HER2(Human Epidermal growth fac..
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cDNA synthesis and RT-PCR
6???
?? : Testis ?????? ??? RNA? cDNA? ???? PCR? ?? ???? ? ????? ?? ???? testis ??? target RNA? ????? ????. ?? * Reverse Transcription PCR RT-PCR? ??? ?? ?? RNA? ???? ?? ????. ???? intron? exon? ??.. -
cDNA
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I.Introduction RNA? single strand? ???? ???? ??? ????? cDNA? ??? ??? ?? ????. RNA ratio DNA? RNA? 260nm?? ?? ?? ?? ????, ???? 280nm?? ?? ?? ?? ????. RNA/??? ??? ?? ?? ??? ??? ? ??.A260/A280 ??? ???..
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???A+/PCR ?????
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1. ????(Title) : Polymerase Chain Reaction(PCR) 2. ?? ? ??(Principle) 1) PCR? ?? ??? ?? ??(genome)?? ????? ?? ???? ???? ??? ???? ???? ???? ???? ???? ???. ??? PCR??? ???? ?? ?? ?? DNA??? DNA? ????..
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- [???] ??????? PCR and Restriction enzyme digestion ????? A0
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- [???] ??????? PCR and Restriction enzyme digestion ????? A0
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- [A+ ???] - PCR (???????)
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- [A+???]??????1 - ????(Restriction enzymes? ??? ????? ??? ????????(PCR)
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- [???????]Competent cell? ?? ?????? ?? ??? PCR product? ????
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- ????????? Real-time PCR (Quantitative RT-PCR) ?????
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- [???????]Quantitative Real Time Polymerase Chain Reaction(qRT-PCR)
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?? ??? ????? EasyAI
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