GOLD
GOLD ๋“ฑ๊ธ‰์˜ ํŒ๋งค์ž ์ž๋ฃŒ

์„œ๊ฐ•๋Œฟ’ํ•™๊ป“ ํ˜„๋Œ€์ƒ๋ฌผํ•™์”„ํ—˜3 ์„ธํฌ์˜ ๊ณ„๋Œ€๋ฐฐ์–‘, ์ง„ํ•ต์„ธํฌ์˜ DNA Damage & Repair ๋ฐ Marker ํ™•์ธ

"์„œ๊ฐ•๋Œฟ’ํ•™๊ป“ ํ˜„๋Œ€์ƒ๋ฌผํ•™์”„ํ—˜3 ์„ธํฌ์˜ ๊ณ„๋Œ€๋ฐฐ์–‘, ์ง„ํ•ต์„ธํฌ์˜ DNA Damage & Repair ๋ฐ Marker ํ™•์ธ"์— ๋Œ€ํ•œ ๋‚ด์šฉ์ž…๋‹ˆ๋‹ค.
19 ํŽ˜์ด์งฟ’
์›Œ๋“œ
์ตœ์ดˆ๋“ฑ๋ก์ผ 2022.01.27 ์ตœ์ข…์ ฟ’์ž‘์ผ 2021.10
19P ๋ฏธ๋้ฉ๋ณด๊ธฐ
์„œ๊ฐ•๋Œฟ’ํ•™๊ป“ ํ˜„๋Œ€์ƒ๋ฌผํ•™์”„ํ—˜3 ์„ธํฌ์˜ ๊ณ„๋Œ€๋ฐฐ์–‘, ์ง„ํ•ต์„ธํฌ์˜ DNA Damage & Repair ๋ฐ Marker ํ™•์ธ
  • ๋ฏธ๋้ฉ๋ณด๊ธฐ

    ์†Œ๊ฐœ

    "์„œ๊ฐ•๋Œฟ’ํ•™๊ป“ ํ˜„๋Œ€์ƒ๋ฌผํ•™์”„ํ—˜3 ์„ธํฌ์˜ ๊ณ„๋Œ€๋ฐฐ์–‘, ์ง„ํ•ต์„ธํฌ์˜ DNA Damage & Repair ๋ฐ Marker ํ™•์ธ"์— ๋Œ€ํ•œ ๋‚ด์šฉ์ž…๋‹ˆ๋‹ค.

    ๋ชฉ์ฐจ

    1. Abstract
    2. Introduction
    3. Materials and Methods
    4. Data & Results
    5. Discussion
    6. References

    ๋ณธ๋ๅฉ๋‚ด์šฉ

    ์ด๋ฒˆ ์‹คํ—˜์—์„œ๋Š” ์ง„ํ•ต์„ธํฌ์ธ HaCaT cell์„ ์„ธํฌ์˜ ๊ณ„๋Œ€๋ฐฐ์–‘์„ ํ†ตํ•ด Incubationํ•˜๊ณ , ์„ธํฌ์˜ ๊ฐœ์ˆ˜๋ฅผ ๊ณ„์‚ฐํ•ด๋ณด์•˜์œผ๋ฉฐ, ์ด ์„ธํฌ๋ฅผ Doxorubicin Damaging agent๋ฅผ ์ฒ˜๋ฆฌํ•ด, Western blot๊ณผ ICC(Immunocytochemistry)๋ฐฉ๋ฒ•์„ ์‚ฌ์šฉํ•ด Doxorubicin์˜ ์œ ๋ฌด์— ๋”ฐ๋ฅธ Cell์˜ ๊ฒฝํ–ฅ์„ ์•Œ์•„๋ณด์•˜๋‹ค. ์„ฑ์ธ ํ”ผ๋ถ€์—์„œ์˜ ๋ถˆ๋ฉธ์„ธํฌ์ธ HaCaT cell์€ In vitro์—์„œ ์ž๋ฐœ์ ์œผ๋กœ ๋ณ€ํ˜•๋˜๋Š” Cell์ด๋ฉฐ, ๋ฐ”๋‹ฅ์—์„œ ์ž๋ผ๋Š” Adherent growing Cell์ด๋‹ค. Doxorubicin์€ ๋ฐ์€ ํ˜•๊ด‘์„ ๋ ๋ฉฐ, ํ•ญ์•”์ œ๋กœ ์“ฐ์ด๋ฉฐ, DNA damaging agent์ด๋‹ค. Serine139์˜ Histone H2AX์˜ Phosphorylation์„ ํ†ตํ•œ Cell์˜ ์กฐ์ž‘์„ ์ง„ํ–‰ํ•œ๋‹ค. ษค-H2AX๋Š” H2AX์˜ Phosphorylated form์ด๋ฉฐ, Repair protein์„ ๋ชจ์œผ๊ณ , DNA damage response foci์˜ Integrity๋ฅผ ์œ ์ง€ํ•œ๋‹ค. ๊ณ„๋Œ€๋ฐฐ์–‘์„ ํ†ตํ•œ Cell์˜ ๋ฐ€๋„๋Š” 5.015*104 Cells/mL๋กœ ๊ณ„์‚ฐ๋˜์—ˆ์œผ๋ฉฐ, Western blot์˜ ๊ฒฐ๊ณผ๋กœ๋Š” Doxorubicin์ฒ˜๋ฆฌํ•œ ๋ถ€๋ถ„์—์„œ ษค-H2AX์ด ์ฒ˜๋ฆฌํ•˜์ง€ ์•Š์€ ๋ถ€๋ถ„๋ณด๋‹ค ์„ ๋ช…ํžˆ ๋‚˜์™”์œผ๋ฉฐ, ICC์˜ ๊ฒฐ๊ณผ๋กœ Control์—์„œ๋Š” DAPI์ฒ˜๋ฆฌํ•œ ๋ถ€๋ถ„์ด ํ‘ธ๋ฅธ์ƒ‰์„ ๋ ์—ˆ๊ณ , FITC์˜ ๊ฒฝ์šฐ, ์•„๋ฌด๋Ÿฐ ์ƒ‰์„ ๋ ์ง€ ์•Š์•˜๋‹ค. Doxorubicin์„ ์ฒ˜๋ฆฌํ•œ Cell์„ ๊ด€์ฐฐํ•œ ๊ฒฐ๊ณผ, DAPI์™ฟ’ FITC๋ฅผ ์ฒ˜๋ฆฌํ•œ Cell์—์„œ ํ‘ธ๋ฅธ์ƒ‰๊ณผ ๋…น์ƒ‰์œผ๋กœ ๋น›๋‚˜๋Š” ๊ฒƒ์ด ๊ด€์ฐฐ๋˜์—ˆ์œผ๋ฉฐ, Cell์˜ ์œ„์น˜ ๋˜ํ•œ ์ผ์น˜ํ–ˆ๋‹ค. ์ด๋ฅผ ํ†ตํ•ด DNA์˜ ์†์ƒ๊ณผ ์ˆ˜๋ฆฌ ๊ณผ์ •์„ ์•Œ ์ˆ˜ ์žˆ์—ˆ๋‹ค. ์‹คํ—˜์— ์‚ฌ์šฉ๋œ Gamma H2AX์ด์™ธ์˜ DNA Damaging agent marker์™ฟ’ Doxorubicin์ด์™ธ์— ์‚ฌ์šฉ๋  ์ˆ˜ ์žˆ๋Š” ๋ฌผ์งˆ์„ ์•Œ์•„๋ณด์•˜๊ณ , Doxorubicin๊ณผ Tubulin์ด ์‚ฌ์šฉ๋œ ์ด์œ ๋ฅผ ํ†ตํ•ด ์ž‘์šฉ๊ธฐ์ „์„ ์•Œ์•„๋ณด์•˜๋‹ค. Control sample์—์„œ๋„ Gamma H2AX๊ฐ€ ๋ฐœํ˜„๋œ ์ด์œ ๋ฅผ ์•Œ์•„๋ณด์•„ ์‹คํ—˜์„ ๋งˆ๋ฌด๋ฆฌํ•˜์˜€๋‹ค.

    ์ฐธ๊ณ ์ž๋ฃŒ

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    ยท ร–lschlรคger, Veronika; Schrader, Andreas; Hockertz, Stefan (13 December 2011). "Comparison of Primary Human Fibroblasts and Keratinocytes with Immortalized Cell Lines Regarding their Sensitivity to Sodium Dodecyl Sulfate in a Neutral Red Uptake Cytotoxicity Assay". Arzneimittelforschung. 59 (3): 146โ€“152. doi:10.1055/s-0031-1296378. PMID 19402346.
    ยท Pang B, Qiao X, Janssen L, Velds A, Groothuis T, Kerkhoven R, Nieuwland M, Ovaa H, Rottenberg S, van Tellingen O, Janssen J, Huijgens P, Zwart W, Neefjes J (2013). "Drug-induced histone eviction from open chromatin contributes to the chemotherapeutic effects of doxorubicin". Nature Communications. 4 (5): 1908. Bibcode:2013NatCo...4.1908P. doi:10.1038/ncomms2921. PMC 3674280. PMID 23715267.
    ยท Pang B, Qiao X, Janssen L, Velds A, Groothuis T, Kerkhoven R, Nieuwland M, Ovaa H, Rottenberg S, van Tellingen O, Janssen J, Huijgens P, Zwart W, Neefjes J (2013). "Drug-induced histone eviction from open chromatin contributes to the chemotherapeutic effects of doxorubicin". Nature Communications. 4 (5): 1908. Bibcode:2013NatCo...4.1908P. doi:10.1038/ncomms2921. PMC 3674280. PMID 23715267.
    ยท Wikipedia - Fvasconcellos 23:30, 4 May 2007 (UTC) - Own work
    ยท Jakob B, Splinter J, Conrad S, Voss KO, Zink D, Durante M, Lรถbrich M, Taucher-Scholz G (August 2011). "DNA double-strand breaks in heterochromatin elicit fast repair protein recruitment, histone H2AX phosphorylation and relocation to euchromatin". Nucleic Acids Research. 39 (15): 6489โ€“99. doi:10.1093/nar/gkr230. PMC 3159438. PMID 21511815.
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    ยท DNA DAMAGE REPAIR MARKERS, proteintechยฎ Inc
    ยท Weiss RB (December 1992). "The anthracyclines: will we ever find a better doxorubicin?". Seminars in Oncology. 19 (6): 670โ€“86. PMID 1462166.
    ยท Tacar, O; Sriamornsak, P; Dass, CR (February 2013). "Doxorubicin: an update on anticancer molecular action, toxicity and novel drug delivery systems". The Journal of Pharmacy and Pharmacology. 65 (2): 157โ€“70. doi:10.1111/j.2042-7158.2012.01567.x. PMID 23278683. S2CID 34737360.
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    ยท Johnstone TC, Suntharalingam K, Lippard SJ (March 2016). "The Next Generation of Platinum Drugs: Targeted Pt(II) Agents, Nanoparticle Delivery, and Pt(IV) Prodrugs". Chemical Reviews. 116 (5): 3436โ€“86. doi:10.1021/acs.chemrev.5b00597. PMC 4792284. PMID 26865551.
    ยท "Cisplatin". The American Society of Health-System Pharmacists. Archived from the original on 21 December 2016. Retrieved 8 December 2016.
  • Easy Ai ์š”์•ฝ

    ์ด ๋ฌธ์„œ๋Š” HaCaT ์„ธํฌ์˜ ๊ณ„๋Œ€๋ฐฐ์–‘ ๊ณผ์ •๊ณผ DNA ์†์ƒ ์œ ๋ฐœ ์‹คํ—˜์„ ํ†ตํ•ด DNA ์†์ƒ marker์ธ ฮณ-H2AX์˜ ๋ฐœํ˜„ ์–‘์ƒ์„ Western blot๊ณผ ICC ๊ธฐ๋ฒ•์œผ๋กœ ๊ด€์ฐฐํ•˜๊ณ  ๋ถ„์„ํ•œ ๋‚ด์šฉ์„ ์ƒ์„ธํžˆ ๊ธฐ์ˆ ํ•˜๊ณ  ์žˆ์Šต๋‹ˆ๋‹ค. ์‹คํ—˜์—์„œ๋Š” DNA ์†์ƒ ์œ ๋ฐœ ๋ฌผ์งˆ์ธ Doxorubicin์„ ์ฒ˜๋ฆฌํ•˜์—ฌ ์„ธํฌ ๋‚ด DNA ์†์ƒ์„ ์œ ๋„ํ•˜๊ณ , ์ด์— ๋Œ€ํ•œ ์„ธํฌ์˜ ๋ฐ˜์‘๊ณผ ๋ณต๊ตฌ ๊ณผ์ •์„ ํ™•์ธํ•˜์˜€์Šต๋‹ˆ๋‹ค. Western blot ๊ฒฐ๊ณผ, Doxorubicin ์ฒ˜๋ฆฌ ์‹œ ฮณ-H2AX์˜ ๋ฐœํ˜„์ด ์ฆ๊ฐ€ํ•˜๋Š” ๊ฒƒ์„ ๊ด€์ฐฐํ•  ์ˆ˜ ์žˆ์—ˆ์œผ๋ฉฐ, ICC ๊ฒฐ๊ณผ์—์„œ๋„ Doxorubicin ์ฒ˜๋ฆฌ ์„ธํฌ์—์„œ ฮณ-H2AX์˜ ๋ฐœํ˜„์ด ๊ด€์ฐฐ๋˜์—ˆ์Šต๋‹ˆ๋‹ค. ์ด๋ฅผ ํ†ตํ•ด Doxorubicin์ด DNA ์†์ƒ์„ ์œ ๋ฐœํ•˜๊ณ  ์ด์— ๋Œ€ํ•œ ์„ธํฌ์˜ ๋ณต๊ตฌ ๋ฐ˜์‘์ด ์ผ์–ด๋‚จ์„ ํ™•์ธํ•  ์ˆ˜ ์žˆ์—ˆ์Šต๋‹ˆ๋‹ค. ๋˜ํ•œ DNA ์†์ƒ marker๋กœ ํ™œ์šฉ๋  ์ˆ˜ ์žˆ๋Š” ๋‹ค๋ฅธ ๋ฌผ์งˆ๋“ค์— ๋Œ€ํ•ด์„œ๋„ ์‚ดํŽด๋ณด์•˜์œผ๋ฉฐ, Doxorubicin๊ณผ Tubulin์˜ ์‚ฌ์šฉ ์ด์œ ์™ฟ’ ์ž‘์šฉ ๊ธฐ์ „์— ๋Œ€ํ•ด ๋ถ„์„ํ•˜์˜€์Šต๋‹ˆ๋‹ค. ๋งˆ์ง€๋ง‰์œผ๋กœ Control ์ƒ˜ํ”Œ์—์„œ๋„ ฮณ-H2AX๊ฐ€ ๋ฐœํ˜„๋œ ์ด์œ ์— ๋Œ€ํ•ด ๊ณ ์ฐฐํ•˜์˜€์Šต๋‹ˆ๋‹ค. ์ด๋ฒˆ ์‹คํ—˜์„ ํ†ตํ•ด ์ง„ํ•ต์„ธํฌ์˜ DNA ์†์ƒ ๋ฐ ๋ณต๊ตฌ ๊ณผ์ •์— ๋Œ€ํ•œ ์ดํ•ด๋ฅผ ๋†’์ผ ์ˆ˜ ์žˆ์—ˆ์œผ๋ฉฐ, ๊ด€๋ จ ์—ฐ๊ตฌ์— ํ™œ์šฉํ•  ์ˆ˜ ์žˆ๋Š” ์œ ์˜๋ฏธํ•œ ๊ฒฐ๊ณผ๋ฅผ ๋„์ถœํ•˜์˜€์Šต๋‹ˆ๋‹ค.
  • ์ž๋ฃŒํ›„๊ธฐ

    Ai ๋ฆฌ๋ทฐ
    ์ด ๋ฌธ์„œ๋Š” HaCaT ์„ธํฌ๋ฅผ ์ด์šฉํ•œ ๊ณ„๋Œ€๋ฐฐ์–‘, DNA ์†์ƒ ์œ ๋ฐœ ๋ฐ ๋ณต๊ตฌ ๊ณผ์ •์„ Western blot๊ณผ ICC ๊ธฐ๋ฒ•์„ ํ†ตํ•ด ๋ฉด๋ฐ€ํžˆ ๋ถ„์„ํ•œ ๋‚ด์šฉ์„ ๋‹ด๊ณ  ์žˆ์Šต๋‹ˆ๋‹ค.
    • ์ž์ฃผ๋ฌป๋Š”์งˆ๋ๅฉ์˜ ๋‹ต๋ณ€์„ ํ™•์ธํ•ด ์ฃผ์„ธ์š”

      ํ•ดํ”ผ์บ ํผ์Šค FAQ ๋”๋ต–๊ธฐ

      ๊ผญ ์•Œ์•„์ฃผ์„ธ์š”

      • ์ž๋ฃŒ์˜ ์ •๋ณด ๋ฐ ๋‚ด์šฉ์˜ ์ง„์‹ค์„ฑ์— ๋Œ€ํ•˜์—ฌ ํ•ดํ”ผ์บ ํผ์Šค๋Š” ๋ณด์ฆํ•˜์ง€ ์•Š์œผ๋ฉฐ, ํ•ด๋‹น ์ •๋ณด ๋ฐ ๊ฒŒ์‹œ๋ฌผ ์ €์ž‘๊ถŒ๊ณผ ๊ธฐํƒ€ ๋ฒ•์  ์ฑ…์ž„์€ ์ž๋ฃŒ ๋“ฑ๋ก์ž์—๊ฒŒ ์žˆ์Šต๋‹ˆ๋‹ค.
        ์ž๋ฃŒ ๋ฐ ๊ฒŒ์‹œ๋ฌผ ๋‚ด์šฉ์˜ ๋ถˆ๋ฒ•์  ์ด์šฉ, ๋ฌด๋‹จ ์ „์žฌโˆ™๋ฐฐํฌ๋Š” ๊ธˆ์ง€๋˜์–ด ์žˆ์Šต๋‹ˆ๋‹ค.
        ์ €์ž‘๊ถŒ์นจํ•ด, ๋ช…์˜ˆํ›ผ์† ๋“ฑ ๋ถ„์Ÿ ์š”์†Œ ๋ฐœ๊ฒฌ ์‹œ ๊ณ ๊ฐ๋น„๋ฐ”์นด์ง€๋…ธ Viva์˜ ์ €์ž‘๊ถŒ์นจํ•ด ์‹ ๊ณ ๋น„๋ฐ”์นด์ง€๋…ธ Viva๋ฅผ ์ด์šฉํ•ด ์ฃผ์‹œ๊ธฐ ๋ฐ”๋ž๋‹ˆ๋‹ค.
      • ํ•ดํ”ผ์บ ํผ์Šค๋Š” ๊ตฌ๋งค์ž์™ฟ’ ํŒ๋งค์ž ๋ชจ๋‘๊ฐ€ ๋งŒ์กฑํ•˜๋Š” ์„œ๋น„์Šค๊ฐ€ ๋˜๋„๋ก ๋…ธ๋ ฅํ•˜๊ณ  ์žˆ์œผ๋ฉฐ, ์•„๋ž˜์˜ 4๊ฐ€์ง€ ์ž๋ฃŒํ™˜๋ถˆ ์กฐ๊ฑด์„ ๊ผญ ํ™•์ธํ•ด์ฃผ์‹œ๊ธฐ ๋ฐ”๋ž๋‹ˆ๋‹ค.
        ํŒŒ์ผ์˜ค๋ฅ˜ ์ค‘๋ณต์ž๋ฃŒ ์ €์ž‘๊ถŒ ์—†์Œ ์„ค๋ช…๊ณผ ์‹ค์ œ ๋‚ด์šฉ ๋ถˆ์ผ์น˜
        ํŒŒ์ผ์˜ ๋‹ค์šด๋กœ๋“œ๊ฐ€ ์ œ๋Œ€๋กœ ๋˜์ง€ ์•Š๊ฑฐ๋‚˜ ํŒŒ์ผํ˜•์‹์— ๋งž๋Š” ํ”„๋กœ๊ทธ๋žจ์œผ๋กœ ์ •์ƒ ์ž‘๋™ํ•˜์ง€ ์•Š๋Š” ๊ฒฝ์šฐ ๋‹ค๋ฅธ ์ž๋ฃŒ์™ฟ’ 70% ์ด์ƒ ๋‚ด์šฉ์ด ์ผ์น˜ํ•˜๋Š” ๊ฒฝ์šฐ (์ค‘๋ณต์ž„์„ ํ™•์ธํ•  ์ˆ˜ ์žˆ๋Š” ๊ทผ๊ฑฐ ํ•„์š”ํ•จ) ์ธํ„ฐ๋„ท์˜ ๋‹ค๋ฅธ ์‚ฌ์ดํŠธ, ์—ฐ๊ตฌ๊ธฐ๊ด€, ํ•™๊ป“, ์„œ์  ๋“ฑ์˜ ์ž๋ฃŒ๋ฅผ ๋„์šฉํ•œ ๊ฒฝ์šฐ ์ž๋ฃŒ์˜ ์„ค๋ช…๊ณผ ์‹ค์ œ ์ž๋ฃŒ์˜ ๋‚ด์šฉ์ด ์ผ์น˜ํ•˜์ง€ ์•Š๋Š” ๊ฒฝ์šฐ
    ๋ฌธ์„œ ์ดˆ์•ˆ์„ ์ƒ์„ฑํ•ด์ฃผ๋Š” EasyAI
    ์•ˆ๋…•ํ•˜์„ธ์š”. ํ•ดํ”ผ์บ ํผ์Šค์˜ ๋ฐฉ๋Œ€ํ•œ ์ž๋ฃŒ ์ค‘์—์„œ ์„ ๋ณ„ํ•˜์—ฌ ๋‹น์‹ ๋งŒ์˜ ์ดˆ์•ˆ์„ ๋งŒ๋“ค์–ด์ฃผ๋Š” EasyAI ์ž…๋‹ˆ๋‹ค.
    ์ €๋Š” ์•„๋ž˜์™ฟ’ ๊ฐ™์ด ์ž‘์—…์„ ๋„์™ฟ’๋“œ๋ฆฝ๋‹ˆ๋‹ค.
    - ์ฃผ์ œ๋งŒ ์ž…๋ ฅํ•˜๋ฉด ๋ชฉ์ฐจ๋ถ€ํ„ฐ ๋ณธ๋ๅฉ๋‚ด์šฉ๊นŒ์ง€ ์ž๋™ ์ƒ์„ฑํ•ด ๋“œ๋ฆฝ๋‹ˆ๋‹ค.
    - ์žฅ๋ฌธ์˜ ์ฝ˜ํ…์ธ ๋ฅผ ์‰ฝ๊ณ  ๋น ๋ฅด๊ฒŒ ์ž‘์„ฑํ•ด ๋“œ๋ฆฝ๋‹ˆ๋‹ค.
    - ์Šคํ† ์–ด์—์„œ ๋ฌด๋ฃŒ ์บ์‹œ๋ฅผ ๊ณ„์ •๋ณ„๋กœ 1ํšŒ ๋ฐœ๊ธ‰ ๋ฐ›์„ ์ˆ˜ ์žˆ์Šต๋‹ˆ๋‹ค. ์ง€๊ธˆ ๋ฐ”๋กœ ์ฒดํ—˜ํ•ด ๋ณด์„ธ์š”!
    ์ด๋Ÿฐ ์ฃผ์ œ๋“ค์„ ์ž…๋ ฅํ•ด ๋ณด์„ธ์š”.
    - ์œ ์•„์—๊ฒŒ ์ ํ•ฉํ•œ ๋ฌธํ•™์ž‘ํ’ˆ์˜ ๊ธฐ์ค€๊ณผ ํŠน์„ฑ
    - ํ•œ๊ตญ์ธ์˜ ๊ฐ€์น˜๊ด€ ์ค‘์—์„œ ์ •์‹ ์  ๊ฐ€์น˜๊ด€์„ ์ด๋ฃจ๋Š” ๊ฒƒ๋“ค์„ ๋ฌธํ™”์  ๋ฌธ๋ฒ•์œผ๋กœ ์ •๋ฆฌํ•˜๊ณ , ํ˜„๋Œ€ํ•œ๊ตญ์‚ฌํšŒ์—์„œ ์ผ์–ด๋‚˜๋Š” ์‚ฌ๊ฑด๊ณผ ์‚ฌ๊ณ ๋ฅผ ๋น„๊ตํ•˜์—ฌ ์ž์‹ ์˜ ์˜๊ฒฌ์œผ๋กœ ๊ธฐ์ˆ ํ•˜์„ธ์š”
    - ์ž‘๋ณ„์ธ์‚ฌ ๋…ํ›„๊ฐ
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    ์ฑ—๋ด‡์œผ๋กœ ๊ฐ„ํŽธํ•˜๊ฒŒ ์ƒ๋‹ดํ•ด๋ณด์„ธ์š”.
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    ์•ˆ๋…•ํ•˜์„ธ์š”. ํ•ดํ”ผ์บ ํผ์Šค AI ์ฑ—๋ด‡์ž…๋‹ˆ๋‹ค. ๋ฌด์—‡์ด ๊ถ๊ธˆํ•˜์‹ ๊ฐ€์š”?
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