DIAMOND
DIAMOND ๋“ฑ๊ธ‰์˜ ํŒ๋งค์ž ์ž๋ฃŒ

์„œ๊ฐ•๋Œฟ’ ํ™”๊ณต์ƒ๋ช…๊ณตํ•™์‹คํ—˜1 A+ < Enzyme kinetics > ๋ ˆํฌํŠธ

Pre์™ฟ’ Main ๋ ˆํฌํŠธ๊ฐ€ ํ•ฉ์ณ์ง„ ํ†ตํ•ฉ๋ณธ์ž…๋‹ˆ๋‹ค. ๋ ˆํฌํŠธ ์ด์  1๋“ฑ์œผ๋กœ "A+" ๋ฐ›์•˜์Šต๋‹ˆ๋‹ค. ํ•ด๋‹น ๋ ˆํฌํŠธ ๊ฐ์ ์—†์ด ๋งŒ์  ๋ฐ›์•˜์Šต๋‹ˆ๋‹ค.
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์ตœ์ดˆ๋“ฑ๋ก์ผ 2024.01.24 ์ตœ์ข…์ ฟ’์ž‘์ผ 2022.11
28P ๋ฏธ๋้ฉ๋ณด๊ธฐ
์„œ๊ฐ•๋Œฟ’ ํ™”๊ณต์ƒ๋ช…๊ณตํ•™์‹คํ—˜1 A+ &lt; Enzyme kinetics &gt; ๋ ˆํฌํŠธ
  • ๋ฏธ๋้ฉ๋ณด๊ธฐ

    ์†Œ๊ฐœ

    Pre์™ฟ’ Main ๋ ˆํฌํŠธ๊ฐ€ ํ•ฉ์ณ์ง„ ํ†ตํ•ฉ๋ณธ์ž…๋‹ˆ๋‹ค.
    ๋ ˆํฌํŠธ ์ด์  1๋“ฑ์œผ๋กœ "A+" ๋ฐ›์•˜์Šต๋‹ˆ๋‹ค.
    ํ•ด๋‹น ๋ ˆํฌํŠธ ๊ฐ์ ์—†์ด ๋งŒ์  ๋ฐ›์•˜์Šต๋‹ˆ๋‹ค.

    ๋ชฉ์ฐจ

    <Pre-Report>
    1. ์‹คํ—˜ ๋ชฉ์ 
    2. ๋ฐฐ๊ฒฝ ์ด๋ก 
    3. References

    <Main Report>
    1. ์‹คํ—˜ ๋ฐฉ๋ฒ•
    2. Data & Results
    3. Discussion
    4. Conclusion
    5. References

    ๋ณธ๋ๅฉ๋‚ด์šฉ

    1. ์‹คํ—˜ ๋ชฉ์ 
    Michaelis-Menten ๋ฐฉ์ •์‹์„ ํ†ตํ•˜์—ฌ ํšจ์†Œ์˜ ๋ฉ”์ปค๋‹ˆ์ฆ˜๊ณผ ๋ฐ˜์‘ ์†๋„๋ก ์„ ์ดํ•ดํ•œ๋‹ค. ๊ฒฝ์Ÿ์  ์ €ํ•ด์ œ์˜ ์ฒจ๊ฐ€์— ๋”ฐ๋ฅธ KM, KI์˜ ๋ณ€ํ™”๋ฅผ ์•Œ์•„๋ณธ๋‹ค.
    2. ๋ฐฐ๊ฒฝ ์ด๋ก 
    2.1 ํšจ์†Œ (Enzyme)
    ํšจ์†Œ๋Š” ์ƒ๋ช…์ฒด ๋‚ด์—์„œ ๊ฐ€์žฅ ํƒ์›”ํ•œ ์ด‰๋งค์ด๋‹ค. ์ด‰๋งค๋Š” ๋ฐ˜์‘์˜ ํ™œ์„ฑํ™” ์—๋„ˆ์ง€๋ฅผ ๋‚ฎ์ถ”์–ด ๋ฐ˜์‘์†๋„๋ฅผ ์ƒ์Šน์‹œํ‚ค๋Š” ํ™”ํ•™๋ฌผ์งˆ๋กœ, ๋ฐ˜์‘ ๊ณผ์ •์—์„œ ๋ณ€ํ•˜๊ฑฐ๋‚˜ ์†Œ๋ชจ๋˜์ง€ ์•Š๋Š”๋‹ค. ํšจ์†Œ๋Š” ์ƒ๋ช…์ฒด์˜ ์ค‘์š”ํ•œ ํ™”ํ•™ ๋ฐ˜์‘๋“ค์ด ์•Œ๋งž์€ ์†๋„๋กœ ์ผ์–ด๋‚˜๊ฒŒ๋” ๋•๋Š”๋‹ค. ํšจ์†Œ๋Š” ํŠน์ด์„ฑ์ด ๊ฐ•ํ•˜์—ฌ ๋Œ€๊ฒŒ ํ•˜๋‚˜์˜ ๋‹จ์ผ ํ™”ํ•™๋ฐ˜์‘์ด๋‚˜ ๋ฐ€์ ‘ํžˆ ๊ด€๋ จ๋œ ์ผ๋ จ์˜ ๋ฐ˜์‘๋“ค์„ ์ด‰๋งคํ•œ๋‹ค. ๋‹จ๋ฐฑ์งˆ๋กœ ๊ตฌ์„ฑ๋œ ํšจ์†Œ๋Š” ๋ฐ˜์‘๋ฌผ ๊ธฐ์งˆ์„ ์„ ํƒํ•˜๋Š”๋ฐ ์žˆ์–ด ๋งค์šฐ ๋†’์€ ํŠน์ด์„ฑ์„ ๋ณด์ธ๋‹ค. ๋Œ€๋ถ€๋ถ„์˜ ํšจ์†Œ๋Š” ํ™œ์„ฑ์„ ๊ฐ–๊ธฐ ์œ„ํ•ด ๋ณด์กฐ ์ธ์ž๊ฐ€ ํ•„์š”ํ•˜๋‹ค. ๋ณด์กฐ ์ธ์ž์˜ ์ข…๋ฅ˜์—๋Š” ๊ธˆ์† ์ด์˜จ (Ca2+, Mg2+, Cu2+ ๋“ฑ)๊ณผ ์œ ๊ธฐ๋ฌผ์งˆ (NAD, FAD ๋“ฑ) ๋“ฑ์ด ์žˆ๋‹ค.

    <์ค‘ ๋žต>

    4. Conclusion
    ์ด๋ฒˆ ์‹คํ—˜์—์„œ๋Š” ethanol์˜ ์‚ฐํ™” ๋ฐ˜์‘์„ ์ด‰๋งคํ•˜๋Š” YADH ํšจ์†Œ์— ๋Œ€ํ•œ assay๋ฅผ ์ง„ํ–‰ํ•˜์˜€๋‹ค. YADH ํšจ์†Œ๋Š” ์กฐํšจ์†Œ๋กœ NAD+๊ฐ€ ํ•„์š”ํ•˜๋ฉฐ, NAD+์™ฟ’ ethanol์˜ ๋ฐ˜์‘ ๊ฒฐ๊ณผ๋กœ ethanol์ด ์‚ฐํ™”๋œ๋‹ค. ethanol๊ณผ ๊ตฌ์กฐ์ ์œผ๋กœ ์œ ์‚ฌํ•œ 2,2,2 -trifluoroethanol์„ competitive inhibitor๋กœ์จ ๋„ฃ์–ด ํšจ์†Œ์˜ ์ด‰๋งค ์ž‘์šฉ์— ๋Œ€ํ•œ ์ €ํ•ด์ œ์˜ ์˜ํ–ฅ์„ ํ™•์ธํ•˜๊ณ ์ž ํ•˜์˜€๋‹ค. Lineweaverburk plot์„ ๊ทธ๋ ค Vmax, KM์„ ๊ตฌํ•˜๊ณ , ์ดˆ๊ธฐ์†๋„, Vmax, KM, Kcat์˜ ๋ณ€ํ™”๋ฅผ ํ™•์ธํ•˜์˜€๋‹ค. ์ €ํ•ด์ œ์˜ ๋†๋„๊ฐ€ ์ฆ๊ฐ€ํ•จ์— ๋”ฐ๋ผ Vmax๋Š” ์ผ์ •, KM์€ ์ฆ๊ฐ€, Kcat์€ ๊ฐ์†Œํ•ด์•ผ ํ–ˆ์œผ๋‚˜, ๊ทธ ๊ฒฝํ–ฅ์„ฑ์ด ์ €ํ•ด์ œ ๋†๋„ 0mM์—์„œ 5mM๋กœ ์ฆ๊ฐ€ํ•  ๋•Œ๋Š” ์„ฑ๋ฆฝํ–ˆ์œผ๋‚˜, 5mM์—์„œ 10mM๋กœ ์ฆ๊ฐ€ํ•  ๋•Œ๋Š” ์„ฑ๋ฆฝํ•˜์ง€ ์•Š์•˜๋‹ค. ์ด๋Ÿฌํ•œ ์˜ค์ฐจ๊ฐ€ ๋ฐœ์ƒํ•œ ์›์ธ์— ๋Œ€ํ•œ ๋ถ„์„์„ ์ง„ํ–‰ํ•˜์˜€์œผ๋ฉฐ, ๊ทธ ๊ฒฐ๊ณผ trendline์˜ ๋ถ€์ •ํ™•์„ฑ, ์˜จ๋„ ์ฐจ์ด, ์‹คํ—˜์ž์— ์˜ํ•œ ์˜ค์ฐจ ๋ฐœ์ƒ ๋“ฑ์ด ์›์ธ์œผ๋กœ ๋ถ„์„๋˜์—ˆ๋‹ค.

    ์ฐธ๊ณ ์ž๋ฃŒ

    ยท Taber, L Richard. โ€œThe competitive inhibition of yeast alcohol dehydrogenase by 2,2,2-trifluoroethanolโ€. Biochemical Education 26.3. 1998. 239-242p.
    ยท MK Campbell, SO Farrell. โ€œBiochemistryโ€. 6ed. life science. 2007. 152-165p.
    ยท Kuznetsev AV, Gnaiger E, Laboratory Protocol Lactate Dehydrogenase Cytosolic Marker Enzyme, Oroboros Instrumnets Corp, 2010, p. 8
    ยท S Sarcar, T K Jain, A Maitra, Activity, and stability of yeast alcohol dehydrogenase (YADH) entrapped in aerosol OT reverse micelles, Biotechnol Bioeng, 1992, 20;39(4), p. 474-478.
    ยท Mary K. Campbell, Biochemistry, Life Science, 2015, pp 156-158
  • Easy Ai ์š”์•ฝ

    ์ด ๋ฌธ์„œ๋Š” YADH ํšจ์†Œ๋ฅผ ์ด์šฉํ•œ ethanol ์‚ฐํ™” ๋ฐ˜์‘ ์‹คํ—˜์„ ํ†ตํ•ด ํšจ์†Œ ๋ฐ˜์‘ ์†๋„๋ก ์„ ๋ถ„์„ํ•˜๊ณ ์ž ํ•˜์˜€๋‹ค. ๊ฒฝ์Ÿ์  ์ €ํ•ด์ œ์ธ 2,2,2-trifluoroethanol์„ ์ฒจ๊ฐ€ํ•˜์—ฌ Vmax, KM, Kcat ๋“ฑ์˜ ๋ณ€ํ™”๋ฅผ ๊ด€์ฐฐํ•˜์˜€๋‹ค. ์ €ํ•ด์ œ ๋†๋„ ์ฆ๊ฐ€์— ๋”ฐ๋ผ Vmax๋Š” ์ผ์ •, KM์€ ์ฆ๊ฐ€, Kcat์€ ๊ฐ์†Œํ•˜๋Š” ๊ฒฝํ–ฅ์„ ๋ณด์˜€์œผ๋‚˜, ์ผ๋ถ€ ๊ตฌ๊ฐ„์—์„œ ๊ทธ ๊ฒฝํ–ฅ์„ฑ์ด ์„ฑ๋ฆฝํ•˜์ง€ ์•Š๋Š” ์˜ค์ฐจ๊ฐ€ ๋ฐœ์ƒํ•˜์˜€๋‹ค. ์ด์— ๋Œ€ํ•œ ์›์ธ ๋ถ„์„์„ ์ง„ํ–‰ํ•˜์˜€์œผ๋ฉฐ, ์˜จ๋„ ์ฐจ์ด, ์‹คํ—˜์ž ์˜ค์ฐจ ๋“ฑ์ด ์›์ธ์œผ๋กœ ๋ถ„์„๋˜์—ˆ๋‹ค. ์ด๋ฒˆ ์‹คํ—˜์„ ํ†ตํ•ด ํšจ์†Œ ๋ฐ˜์‘ ์†๋„๋ก ๊ณผ ๊ฒฝ์Ÿ์  ์ €ํ•ด์ œ์˜ ์˜ํ–ฅ์„ ์ดํ•ดํ•  ์ˆ˜ ์žˆ์—ˆ์œผ๋ฉฐ, ์‹คํ—˜ ์„ค๊ณ„์™ฟ’ ๋ฐ์ดํ„ฐ ๋ถ„์„ ์‹œ ๋ฐœ์ƒํ•  ์ˆ˜ ์žˆ๋Š” ์˜ค์ฐจ ์š”์ธ์— ๋Œ€ํ•ด์„œ๋„ ๊ณ ์ฐฐํ•ด๋ณผ ์ˆ˜ ์žˆ์—ˆ๋‹ค. ํ–ฅํ›„ ์‹คํ—˜ ์ ˆ์ฐจ์™ฟ’ ๋ถ„์„ ๋ฐฉ๋ฒ•์„ ๊ฐœ์„ ํ•œ๋‹ค๋ฉด ๋ณด๋‹ค ์ •ํ™•ํ•œ ๊ฒฐ๊ณผ๋ฅผ ์–ป์„ ์ˆ˜ ์žˆ์„ ๊ฒƒ์œผ๋กœ ๊ธฐ๋Œ€๋œ๋‹ค.
  • ์ž๋ฃŒํ›„๊ธฐ

      Ai ๋ฆฌ๋ทฐ
      ํšจ์†Œ ๋ฐ˜์‘์—์„œ ๊ฒฝ์Ÿ์  ์ €ํ•ด์ œ์˜ ์˜ํ–ฅ์„ ํ™•์ธํ•˜๊ณ , Lineweaver-Burk ๋„ํ‘œ๋ฅผ ํ™œ์šฉํ•˜์—ฌ Vmax, KM, Kcat ๋“ฑ์˜ ๋ณ€ํ™”๋ฅผ ๋ถ„์„ํ•˜๊ณ  ์žˆ๋‹ค.
    • ์ž์ฃผ๋ฌป๋Š”์งˆ๋ๅฉ์˜ ๋‹ต๋ณ€์„ ํ™•์ธํ•ด ์ฃผ์„ธ์š”

      ํ•ดํ”ผ์บ ํผ์Šค FAQ ๋”๋ต–๊ธฐ

      ๊ผญ ์•Œ์•„์ฃผ์„ธ์š”

      • ์ž๋ฃŒ์˜ ์ •๋ณด ๋ฐ ๋‚ด์šฉ์˜ ์ง„์‹ค์„ฑ์— ๋Œ€ํ•˜์—ฌ ํ•ดํ”ผ์บ ํผ์Šค๋Š” ๋ณด์ฆํ•˜์ง€ ์•Š์œผ๋ฉฐ, ํ•ด๋‹น ์ •๋ณด ๋ฐ ๊ฒŒ์‹œ๋ฌผ ์ €์ž‘๊ถŒ๊ณผ ๊ธฐํƒ€ ๋ฒ•์  ์ฑ…์ž„์€ ์ž๋ฃŒ ๋“ฑ๋ก์ž์—๊ฒŒ ์žˆ์Šต๋‹ˆ๋‹ค.
        ์ž๋ฃŒ ๋ฐ ๊ฒŒ์‹œ๋ฌผ ๋‚ด์šฉ์˜ ๋ถˆ๋ฒ•์  ์ด์šฉ, ๋ฌด๋‹จ ์ „์žฌโˆ™๋ฐฐํฌ๋Š” ๊ธˆ์ง€๋˜์–ด ์žˆ์Šต๋‹ˆ๋‹ค.
        ์ €์ž‘๊ถŒ์นจํ•ด, ๋ช…์˜ˆํ›ผ์† ๋“ฑ ๋ถ„์Ÿ ์š”์†Œ ๋ฐœ๊ฒฌ ์‹œ ๊ณ ๊ฐ๋น„๋ฐ”์นด์ง€๋…ธ Viva์˜ ์ €์ž‘๊ถŒ์นจํ•ด ์‹ ๊ณ ๋น„๋ฐ”์นด์ง€๋…ธ Viva๋ฅผ ์ด์šฉํ•ด ์ฃผ์‹œ๊ธฐ ๋ฐ”๋ž๋‹ˆ๋‹ค.
      • ํ•ดํ”ผ์บ ํผ์Šค๋Š” ๊ตฌ๋งค์ž์™ฟ’ ํŒ๋งค์ž ๋ชจ๋‘๊ฐ€ ๋งŒ์กฑํ•˜๋Š” ์„œ๋น„์Šค๊ฐ€ ๋˜๋„๋ก ๋…ธ๋ ฅํ•˜๊ณ  ์žˆ์œผ๋ฉฐ, ์•„๋ž˜์˜ 4๊ฐ€์ง€ ์ž๋ฃŒํ™˜๋ถˆ ์กฐ๊ฑด์„ ๊ผญ ํ™•์ธํ•ด์ฃผ์‹œ๊ธฐ ๋ฐ”๋ž๋‹ˆ๋‹ค.
        ํŒŒ์ผ์˜ค๋ฅ˜ ์ค‘๋ณต์ž๋ฃŒ ์ €์ž‘๊ถŒ ์—†์Œ ์„ค๋ช…๊ณผ ์‹ค์ œ ๋‚ด์šฉ ๋ถˆ์ผ์น˜
        ํŒŒ์ผ์˜ ๋‹ค์šด๋กœ๋“œ๊ฐ€ ์ œ๋Œ€๋กœ ๋˜์ง€ ์•Š๊ฑฐ๋‚˜ ํŒŒ์ผํ˜•์‹์— ๋งž๋Š” ํ”„๋กœ๊ทธ๋žจ์œผ๋กœ ์ •์ƒ ์ž‘๋™ํ•˜์ง€ ์•Š๋Š” ๊ฒฝ์šฐ ๋‹ค๋ฅธ ์ž๋ฃŒ์™ฟ’ 70% ์ด์ƒ ๋‚ด์šฉ์ด ์ผ์น˜ํ•˜๋Š” ๊ฒฝ์šฐ (์ค‘๋ณต์ž„์„ ํ™•์ธํ•  ์ˆ˜ ์žˆ๋Š” ๊ทผ๊ฑฐ ํ•„์š”ํ•จ) ์ธํ„ฐ๋„ท์˜ ๋‹ค๋ฅธ ์‚ฌ์ดํŠธ, ์—ฐ๊ตฌ๊ธฐ๊ด€, ํ•™๊ป“, ์„œ์  ๋“ฑ์˜ ์ž๋ฃŒ๋ฅผ ๋„์šฉํ•œ ๊ฒฝ์šฐ ์ž๋ฃŒ์˜ ์„ค๋ช…๊ณผ ์‹ค์ œ ์ž๋ฃŒ์˜ ๋‚ด์šฉ์ด ์ผ์น˜ํ•˜์ง€ ์•Š๋Š” ๊ฒฝ์šฐ

    ํ•จ๊ป˜ ๊ตฌ๋งคํ•œ ์ž๋ฃŒ๋„ ํ™•์ธํ•ด ๋ณด์„ธ์š”!

    ๋ฌธ์„œ ์ดˆ์•ˆ์„ ์ƒ์„ฑํ•ด์ฃผ๋Š” EasyAI
    ์•ˆ๋…•ํ•˜์„ธ์š”. ํ•ดํ”ผ์บ ํผ์Šค์˜ ๋ฐฉ๋Œ€ํ•œ ์ž๋ฃŒ ์ค‘์—์„œ ์„ ๋ณ„ํ•˜์—ฌ ๋‹น์‹ ๋งŒ์˜ ์ดˆ์•ˆ์„ ๋งŒ๋“ค์–ด์ฃผ๋Š” EasyAI ์ž…๋‹ˆ๋‹ค.
    ์ €๋Š” ์•„๋ž˜์™ฟ’ ๊ฐ™์ด ์ž‘์—…์„ ๋„์™ฟ’๋“œ๋ฆฝ๋‹ˆ๋‹ค.
    - ์ฃผ์ œ๋งŒ ์ž…๋ ฅํ•˜๋ฉด ๋ชฉ์ฐจ๋ถ€ํ„ฐ ๋ณธ๋ๅฉ๋‚ด์šฉ๊นŒ์ง€ ์ž๋™ ์ƒ์„ฑํ•ด ๋“œ๋ฆฝ๋‹ˆ๋‹ค.
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