BRONZE
BRONZE ??? ??? ??
[??????2] Transformation
"[??????2] Transformation"? ?? ?????.
6 ???
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????? 2005.03.31
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2005.03
6P ????
![[??????2] Transformation](https://image4.ppt2video.com/Production/thumb212/2005/03/31/data3473497-0001.jpg)
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????
??
1. ????
2. ????
1) Transformation
2) Competent cell
3) Competent cell? ??
(1) Fresh ?? frozen ??? ??? competent cell? ??
(2) Calcium chloride? ??? competent cell? ??
(3) Ultra-competent cell? ??(Inoue method)
4) Electroporation (?????)
5) Selection for transformed cell
(1) Lac selection
(2) ???? ??? selection
(3) DNA ????? ???? ??? ??? ?? 4??
(4) ??????? ????? ???? ? ?? ?? ??
6) ?? ? ??? ??
(1) LB ?? (Luria-Bertani medium) 1 liter
(2) SOC ?? (Terrific Broth)
(3) ???
7) ????? ??? ??
(1) ?? ??? ???? ??
(2) ???? ??? ??
(3) ??? ??? ??
(4) ??? ?? ??
(5) ???? ???? ?? ???? ??? ??
3. ????????
????(transformation)?? ?????? ????? ????? ??? ??? ?? ?? ????? ???? ??? ?? ??? ???? (bacterial transformation)??? ??. ????? ?? ?? ?? ????? ???? ??tm??? ??? ??? ????. ????? ?? ???? ?? ???? ?? ???. ????? ?? ??? ????? ??? ??? ?? ?? ??? ???. ??? ????? ? ?? ?(Bacillus? Streptococcus?)?? ?? ?? ?? ? ? ??? ???? ? ? ??. ???? ??? ???? ????? ???? ???? ??? ?? DNA?? ????. ??? ?? ????? ?????? ??? DNA ????? ???? ?????? ???? ??? ???? ??. ??? ??? ?? ??? competent cell??? ??.
????
¡¤ ? ?????, ????????, ??????, 1997
¡¤ ? ????????, ???? 12?, ??????, 1987
¡¤ ? ???????, ???, ??????? (3?), ???????, 1998
¡¤ ? ?????, David Freifelder, ??????, 1989
¡¤ ? Recombinant DNA ( Watson? 3?, Freeman Publishing, p.73¡«84)
¡¤ ? http://www.kordic.re.kr/%7Etrend/Content302/biology19.html
¡¤ ? http://biochemistry.yonsei.ac.kr/biochem_molecular/??
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????
Ai ????? ??? ?? ??? ?? ?? ??? ???? ??, ?? ????????. ???? ??? ???? ?? ???? ????. ?? ??????. -
??????? ??? ??? ???
? ?????
-
??? ?? ? ??? ???? ??? ?????? ???? ???, ?? ?? ? ??? ???? ?? ?? ??? ?? ????? ????.
?? ? ??? ??? ??? ??, ?? ?????? ???? ????.
?????, ???? ? ?? ?? ?? ? ????? ????? ????? ??? ??? ????. -
?????? ???? ??? ??? ???? ???? ??? ???? ???, ??? 4?? ???? ??? ? ?????? ????.
???? ???? ??? ?? ??? ?? ?? ??? ??? ????? ??? ?? ??? ????? ?? ?????? ?? ???? ?? ?? ?? ??? 70% ?? ??? ???? ?? (???? ??? ? ?? ?? ???) ???? ?? ???, ????, ??, ?? ?? ??? ??? ?? ??? ??? ?? ??? ??? ???? ?? ??
?? ??? ??? ??? ???!
-
[??????]Transformation
5???
1. ???? : 2005? 9? 30? 2. ???? : Transformation 3. ???? : ?E. coli? heat shock??? ?? transformation ??? ??. ? E. coli? transformation ??? ??? ?? ??? ?? ??? transformation? ??? ?? ??, ..
-
????(transformation) ?????
5???
1. Transformation? ?? (1) ????? ?? ????? ????? ??? DNA? ??? ??? ???? ??? ??? ?? ???. ????? 1928? ??? F.????? ?????(·ÎÑ×ëpÇò¾ú)? ???? ??? ??? ??? ?? ?????. ???? ???? ??? ?, ? ??(ÇvĤ)? ?? S?????? DNA? ..
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[????] Transformation of competent E. coli(?? ? ??)
10???
Transformation??? ??? ????? vector? E.coli cell? ???? ??? ? ?? ??. ??? DNA clonning?? ???? ??? ??? ?????? ?? ???? ?? ??? ???? ??? ??. ?? ?? ampicillin-resistance gene? ¦Â-lactamase? encode???, ? ??? ??..
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[??????2] ??????2- ????(transformation)
7???
?? ??? ????? ?? ??? competent cell? ??? ??? heat shock? ?? transform ??? ????. ??? ????? ?? ?? 1?? ¢Ú? Overnight??? ????, ?? ?? ??? competent cell? ??? ?? ???, ??? ?? ?? ??? ???? ?? cell? ??? 2? ???..
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[????] Transformation of competent E. coli
4???
¢Å???? : Transformation of competent E. coli ¢Æ????: Ampicillin? ??? ???? ?? E. coli (XLI-Blue, Amp-r) ? plasmid DNA¡¾pBluescript SK¢ò(+)¡¿? isolation ? ?, ampicillin? ?? ?? ..
???? ??? ?????
?? ?? ??? ???? ???!
-
????
- Alpha7 Nicotinic Acetylcholine Receptor
- ??? | 11 ???
- 2,000?
??? ????
-
??/??
- ?? ?? (??? ??? ??? ?? ? ?? ??)
- ?????12 | 14 ???
- 2,500?
??? ????
-
????
- [A+] Transformation ?????
- reborn | 15 ???
- 5,000?
??? ????
-
????
- ????? ????(Transformation)
- ?? | 7 ???
- 3,000?
-
????
- ??4 Transformation ?
- LFC94 | 8 ???
- 3,000?
-
????
- ????? ??? (2)
- Kees | 6 ???
- 2,500?
-
????
- [??????? ???] Gene cloning ?? ???
- ?? | 11 ???
- 2,000?
-
??/??
- ?????? ?? ???
- ?? | 7 ???
- 10,000?
-
??/??
- [A0, ?????] ??????????1 - FT-IR (Fourier Transform Infrared Spectroscopy)
- ejrtn4862 | 23 ???
- 4,000?
-
??/??
- [????????? A+] Enzyme activity assay
- *?* | 6 ???
- 2,000?
-
??/??
- ???? ???(???? ??)
- ????? | 5 ???
- 1,500?
??? ????
-
????
- ????? ??? ?? ??? ??? ???? ????, RNA??? ??? ??? ??, ??? ?? ?????.
- ?????? | 11 ???
- 2,500?
-
??/??
- Plasmid/DNA ????
- *?* | 13 ???
- 1,500?
-
??/??
- [????????]Transformation
- CORYHENRY | 5 ???
- 1,000?
?? ??? ????? EasyAI
¡°EasyAI ? ??? ?? ??? ???? ?? ? ????"
