[?????]??? ??

1. ??? ??
1) ???? ???? ????
2) SDS-PAGE? gel ?? ??.
3) Coomassie? Silver Staining? ?? ??? ??
3. ?? ??
????
???? ??? ??? ?? ? ?? ?? ???? ?? -> ???? ??????
SDS-PAGE ( Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis )
: SDS? Polyacrylamide ? ??? ????
SDS-PAGE
Sodium dodecyl sulfate-polyacryamide gel electrophoresis (SDS-PAGE)? ???? ???? ?? ?? monitor??, ??? ???? ???(homogeneity)? ?? ?? ?? ?? ????. SDS-PAGE? ????? ???? ???? ????, ??? ???? ??? ??? ?? ?? ????. SDS-PAGE? ? ?? ??? ?? ??? ???? ?? ?? scale up? ? ??. ??, SDS-PAGE? Isoelectric focusing? ???? 2?? ???? ???(resolution)? ?? ?? ????? ??? gel?? ?? ?? polypeptide? ??? ? ??.??? Blotting method? ???? ???? ????? homology? ??? ? ??. ??? SDS-PAGE? ??? ??? ??? ? ?? ??? ??? ????.
?????, ????? ?? ??? ??? ??, ??, ?? ??? ???? ?(net charges)? ????.System? ???? ???(electrophoretic mobility)? ?? ??, ??, ??? ??? ??? ? ?? ?? ??(native configurations)?? ???? ???? ?? ????.SDS-PAGE(sodium dodecyl sulfate polyacrylamide gel electrophoresis)? ??? ?????? ? ????. ? ??? ?? ???? ????? ??? ?????? ??? ??, ????? ???? ?????? ?? ??? ??? ? ???? ????.
coomassie blue staining
????????? ??? ???? ??? ?? ?? ?? ??? ?? ??? ???? ??? ??? ??, ??? ?? ??, mass spectrometry?? ?? ??? ??? Coomassie Blue dye (R and G types)? ?? ?? ???? ??.