DNA 연구왿� 해석

The ITS (internal transcribed spacer region) extracted from DNA of a plant organism, Allinium fistulosum was amplified through PCR process, which makes a number of copies of genes. After purification of DNA fragments, the restriction enzymes cut specific sites on the DNA and were inserted into a plasmid. The plating experiment proved the presence of DNA insert in the vector of a plasmid. Numerous only blue colonies found in the plating experiment indicated that no colonies contained a cloned PCR fragment. These various techniques and samples used for the DNA experiments emphasize the importance of DNA extraction and molecular cloning methods to maximize reliability and competence of genotyping. A new strand was examined using gel electrophoresis to separate different molecules based on physical characteristics such as size, shape, or charge. The analysis of the gel electrophoresis and the restriction enzyme mapping verified the orientation of the PCR fragment, which was anti-parallel, and the sequence homology using the chromatogram and BLAST interpreted the common ancestor from the similarities of the reference DNA sequences. The results of this study supported the hypothesis; the DNA coding region for the 5.8s ribosomal RNA subunit cloned by several Biotechniques steps would have the same sequence of bases as Allium fistulosum DNA originally had.