Tyrosinase activity inhibition ??

¡¾?? Report¡¿

????: 2007. 05. 18 ??? 1~2??

1. ????: Tyrosinase activity inhibition ??

2. ????

??? ?? ?? ??? ?? tyrosine ?? ??? ?????? tyrosinase? ??? ??? ????. ??? tyrosinase ????? ?? ?????? ????, ??????? ?? ? ??. ??? ??? ??? tyrosinase ??????? ?? ????? ??? ??? ??? ??? ??.

3. ?, ??

<1?>???, ???, ???(?), ???, ???

4. ????

(1) potassium phosphate buffer(0.1M pH6.8)? 500ml? ???. ??? ??L-tyrosine (???=225.2) 10mM?? 10ml? ???.
(2) enzyme? 5370units/mg? ???? stock?? 2685units/ml? ???.
(3) stock??? 20mg/ml? ??? 70%EtOH? 100? ???? final??? 200ug/ml? ???. (20mg/ml? ??? ??? ??? 20ul? ??? 70%EtOH1980ul ? ?? ?? ??? 100? ?????.)

buffer
H2O
solvent
tyrosine
enzyme
control
696
32
8
48
16

712
32
8
48


buffer
H2O
sample
tyrosine
enzyme
sample
696
32
8
48
16

712
32
8
48

(4) ??? microtube? ??? ?? ?? ???.
* ??? ??? ????? sample?? 2?? ??? ???.
(5) ??? microtube ? ???(30¡æ)? ?? 10? ?? ???.
(6) ?? voltex ??? uv-visible spectrometer ? ???? 475nm?? ???? ????.
¡ù ?????? ???? ?????.