Cell Structure_Immunofluorescence[?????]
Cell Structure_Immunofluorescence[?????]???.
?? ?? ?? ????^^Cell Structure_Immunofluorescence
¢ñ. Introduction and Theory
1. Transfection
3 ???
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????? 2008.05.16
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2008.03
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Cell Structure_Immunofluorescence[?????]???.
?? ?? ?? ????^^Cell Structure_Immunofluorescence
¢ñ. Introduction and Theory
1. Transfection??
¢ñ. Introduction and Theory
1. Transfection
a. Transfection : `infection by transformation`
b. Methods
2. Immunostaining
a. cell or tissue staining?? ??
b. immunostaining ??
c. immunostaining protocol
3. HA-tag & EGFP-tag
a. HA-tag (Hemagglutinin tag)
b. EGFP-tag (Enhanced Green Fluorescent Protein tag)
? References????
¢ñ. Introduction and Theory
1. Transfection
a. Transfection : `infection by transformation`
The introduction of a foreign gene into mammalian cells.
Transfection? virus vector? ?? transfer vector? ?? foreign gene? eukaryotic cell? introduction ??? ?? ???. transfection? transformation ? ??? transfection? ?? mammalian cell? target?? ??? ???.
transfected cell? foreign DNA? introduction?? ?? ? properties? ??? ??.
b. Methods
foreign DNA? eukaryotic cell? introduction?? ???? ?? carrier? ??? ? ?? ?? ??? ????.
- calcium phosphate : phosphate ion? ???? ?? HeBS(HEPES-bufferd saline solution)? foreign DNA? ??? calcium chloride solution? ???? calcium ion? phosphate? ???? DNA? ?? precipitate? ????. ? suspension? cell? ????, cell? precipitate? uptake?? ??.????
¡¤ <URL> http://www.rndsystems.com/western_blot_detail_objectname_ImmunostainingProtocol.aspx
¡¤ http://en.wikipedia.org/wiki/Immunostaining
¡¤ http://en.wikipedia.org/wiki/Trasnfection
¡¤ http://www.rndsystems.com/western_blot_detail_objectname_ImmunostainingProtocol.aspx#Rapid??
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I. ????? ?? 1. ??? ?? ?? ¡ì ???? ????? ¡ì ??? ??? ????? ?? ??? ??? ??? ???? ???? ?? ? ??? ??. ? ??? ???? ??? ???? ????? ??? ? ??? ????? ?????? ??? ??. ? ?? ???? ???? ? ??? ?? ..
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Immunofluorescence staining of articular chondrocytes
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Immunofluorescence staining of articular chondrocytes ?? molecule? ?? specific primary antibody? fluorescence dye? conjugate?? ?? secondary antibody? ???? ???? ?? (localization), ?? (distribution),..
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1. ????? (Stereoscopic Microscope) ???? ??? ?? ?? ??? ? ??? ? ?? ?? ??? ?? ??? ???? ?? ?? ??. ?? ?????? ? ?? ????? ???? ???????? ??(¹âÝS)? ???? ??? ???? ?? ? ??. *?? ¢Ù???????? ?? ??? ? 15¡ã? ??? 2??..
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[????]transfection
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I. Transfection? ?? Transfection?? ??? DNA? eukarayotic cell? ???? ?? ???? ???, ????? cell? ????? ??? ??? ??? DNA? ??? ? ?? ??. ???? DNA? ?? supercoiled plasma DNA?? siRNA? ????? ??. - Compete..
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Bradford Assay
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¢ñ. Introduction 1.1. Research goal Standard curve? ???, Unknown sample ? ?? ??? ??? ???? ??? ????. 1.2. Basic Mechanism of the experiment Bradford Assay? Coomassie blue (Bradford dye) ..
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